Detection of IgG1 antibodies in ELISA. MAb 2C11 can be used for coating and as conjugate. MAb 2C11 works in immunohistochemistry on cytostat sections.
Subclass-specific for human IgG1. No allotype restriction. Fc - region specific. Specific antibody titer is 1/300 000 by indirect ELISA
2C11 Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with IgG from human serum.
Allergol. et Immunopathol. 1978, vol 1, pp. 423-426 Allergy and Clin. Immunol. News, 1992, vol.4, 21-25 Cat. # 1G2
+ 4 ﾰC
MAbs 4F4, 5D4, E411, BE5, 4G7, 4H10 and IEF6 in PBS, pH 7.4, 0.1 % sodium azide (NaN3) MAb IEF6 by chromatography on protein G Sepharose XTE4 in 0.15 M NaCl, 10 mM Tris, pH 7.5, 0.05 % sodium azide (NaN3)
MAbs 4F4, 5D4, E411, BE5, 4G7 and 4H10 by chromatography on protein A Sepharose MAb XTE4 by ion exchange chromatography
IgG for MAbs BE5, 4G7 and 4H10 IgG1 for MAb XTE4 and IEF6 IgG2a for MAbs 4F4, 5D4 and E411
MAb E411 class specific for human IgE, Fc-region specific MAb 4F4 - IgE C?3-domain specific MAb 5D4 - IgE C?2-domain specific MAb XTE4 - epsilon chain specific
IgG1 for MAb 2C11
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