Monoclonal mouse anti-human IgE

Cat.# 1E4

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Immunology and serology
  • Storage:
    + 4 ᄚC
  • Presentation:
    PBS, pH 7.4, 0.1 % sodium azide (NaN3)
  • Purification:
    Chromatography on protein G Sepharose
  • Applications:
    Detection of IgG1 antibodies in ELISA. MAb 2C11 can be used for coating and as conjugate. MAb 2C11 works in immunohistochemistry on cytostat sections.
  • Specificity:
    Subclass-specific for human IgG1. No allotype restriction. Fc - region specific. Specific antibody titer is 1/300 000 by indirect ELISA
  • MAbs:
    2C11 Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with IgG from human serum.
  • Other information:
    Allergol. et Immunopathol. 1978, vol 1, pp. 423-426 Allergy and Clin. Immunol. News, 1992, vol.4, 21-25 Cat. # 1G2
  • Storage:
    + 4 ᄚC
  • Presentation:
    MAbs 4F4, 5D4, E411, BE5, 4G7, 4H10 and IEF6 in PBS, pH 7.4, 0.1 % sodium azide (NaN3) MAb IEF6 by chromatography on protein G Sepharose XTE4 in 0.15 M NaCl, 10 mM Tris, pH 7.5, 0.05 % sodium azide (NaN3)
  • Purification:
    MAbs 4F4, 5D4, E411, BE5, 4G7 and 4H10 by chromatography on protein A Sepharose MAb XTE4 by ion exchange chromatography
  • MAb isotypes:
    IgG for MAbs BE5, 4G7 and 4H10 IgG1 for MAb XTE4 and IEF6 IgG2a for MAbs 4F4, 5D4 and E411
  • Specificity:
    MAb E411 class specific for human IgE, Fc-region specific MAb 4F4 - IgE C?3-domain specific MAb 5D4 - IgE C?2-domain specific MAb XTE4 - epsilon chain specific
  • Cat.#
    1E4
  • MAb isotypes:
    IgG1 for MAb 2C11
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Monoclonal mouse anti-human IgE (MAb: )

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