Adn23, Adn27, Adn36, Adn63, Adn94, Adn279, Adn305
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with human recombinant adiponectin from E. coli (MAb Adn23) or with native human adiponectin (MAbs Adn27, Adn36, Adn63, Adn94, Adn279 and Adn305).
Human serum adiponectin
IgG1 for MAbs Adn63, Adn94, Adn279, Adn305cc, Adn305
IgG2a for MAbs Adn23, Adn27, Adn36
MAbs Adn23 and Adn63 react with human serum adiponectin in Western blotting.
MAbs Adn23 and Adn63 recognise the whole spectrum of oligomeric forms of adiponectin after non-heating, non-reducing SDS-PAGE.
Recommended pairs for total adiponectin immunodetection in sandwich immunoassay (capture – detection):
Adn36 – Adn27
Adn63 – Adn94
Adn94 – Adn63
Adn305cc – Adn279
Chromatography on protein A Sepharose
PBS, pH 7.4, 0.09 % sodium azide (NaN3)
+4 °C (+2 … +8 °C allowed)
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.