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Monoclonal mouse anti-human leptin Cat.#

2LE1

Data Sheet

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Properties
Unit

mg

Cat.#

2LE1

MAbs:

3G7, 4F12

Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with recombinant leptin.

Specificity:

MAbs recognize recombinant human leptin and leptin circulating in human blood.

MAb isotypes:

IgG1 for MAbs 3G7, 4F12

Applications:

Leptin immunoassay. All MAbs recognize human leptin in Western blotting.

MAbs were tested in sandwich immunoassay with human blood samples. All recommended pairs recognize antigen in human blood with high sensitivity.

Recommended pairs for sandwich immunoassay (capture - detection):
3G7 - 4F12                   
4F12 - 3G7                                     

Purification:

MAb 4F12: Chromatography on protein A Sepharose and gel filtration, MAb 3G7: Chromatography on protein A Sepharose

Presentation:

PBS, pH 7.4, 0.09 % sodium azide (NaN)

Storage:

+4 °C (+2 … +8 °C allowed)

Material safety note:

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

Additional product information:

Adiponectin TechNotes
Apiponectin product info

References:

Search Leptin references from PubMed.
See other references.
See other Metabolic syndrome products.

Download datasheet as a PDF file below
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