Insulin, human, antibody

Cat.# 2I1

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Metabolic syndrome
  • Cat.#

    2I1

  • MAbs:

    C7C9, D4B8, 7F8, 3A6, 8E2, 7F5

    Hybridoma clones have been derived from hybridization of X63-Ag8-653 myeloma cells with spleen cells of Balb/c mice immunized with purified human insulin.

  • Specificity:

    Insulin, human

    MAb C7C9 reacts with C-terminal pentapeptide of insulin β-chain. MAbs 7F8, D4B8, 3A6 and 8E2
    react with different antigenic determinants.

    These antibodies cross-react with human proinsulin, bovine insulin (30%) and porcine insulin. No cross-reaction with free C-peptide.

  • MAb isotypes:

    IgG1, kappa for all MAbs

  • Applications:

    Detection of insulin in two-site enzyme immunoassay. Following pairs are recommended (capture - detection):
    7F8 - D4B8, 3A6 - 8E2.

    MAb D4B8 can be used in immunohistochemistry on frozen sections.

  • Purification:

    Chromatography on protein A Sepharose

  • Presentation:

    PBS, pH 7.4, 0.09 % sodium azide (NaN₃)

  • Storage:

    +4 °C (+2 … +8 °C allowed)

  • Material safety note:

    This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

    Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

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Insulin, human, antibody (MAb: )

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