C7C9, 7F8, D4B8
Hybridoma clones have been derived from hybridization of X63-Ag8-653 myeloma cells with spleen cells of Balb/c mice immunized with purified human insulin.
MAb C7C9 reacts with C-terminal pentapeptide of insulin β-chain. MAbs 7F8 and D4B8 react with different antigenic determinants.
These antibodies cross-react with human proinsulin, bovine insulin (30%) and porcine insulin. No cross-reaction with free C-peptide.
IgG1, kappa for MAbs C7C9, 7F8, D4B8
Detection of insulin in sandwich immunoassay. Recommended pair (capture-detection): 7F8 - D4B8.
MAb D4B8 can be used in immunohistochemistry on frozen sections.
Protein A chromatography
PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
+4 °C (+2 … +8 °C allowed)
This product is sold for research or further manufacturing use only. Standard Laboratory Practices should be followed when handling this material.
Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
(Catalogue number and MAb: 2I1-)