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Monoclonal mouse anti-human insulin Cat.#

2I1

Data Sheet

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Properties
Unit

mg
Cat.#

2I1
MAbs:

C7C9, D4B8, 7F8, 3A6, 8E2, 7F5

Hybridoma clones have been derived from hybridization of X63-Ag8-653 myeloma cells with spleen cells of Balb/c mice immunized with purified human insulin.
Specificity:

Insulin, human

MAb C7C9 reacts with C-terminal pentapeptide of insulin β-chain. MAbs 7F8, D4B8, 3A6 and 8E2
react with different antigenic determinants.

Kass for MAb 7F8 is 7.5 x 109M⎺ 1
Kass for MAb D4B8 is 1.5 x 108 M⎺1
Kass for MAb 3A6 is 8.0 x 108 M⎺1
Kass for MAb 8E2 is 6.0 x 108 M⎺1
Kass for MAb C7C9 is 1.0 x 109 M⎺1
Kass for MAb 7F5 is 1.0 x 109 M⎺1

These antibodies cross-react with human proinsulin, bovine insulin (30%) and porcine insulin. No cross-reaction with free C-peptide.
MAb isotypes:

IgG1, kappa for all MAbs
Applications:

Detection of insulin in two-site enzyme immunoassay. Following pairs are recommended (capture - detection):
7F8 - D4B8, 3A6 - 8E2.

MAb D4B8 can be used in immunohistochemistry on frozen sections.
Purification:

Chromatography on protein A Sepharose
Presentation:

PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
Storage:

+4 °C (+2 … +8 °C allowed)
Material safety note:

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Additional product information:

Diabetes marker products
Download datasheet as a PDF file below
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