C7C9, D4B8, 7F8, 3A6, 8E2, 7F5
Hybridoma clones have been derived from hybridization of X63-Ag8-653 myeloma cells with spleen cells of Balb/c mice immunized with purified human insulin.
MAb C7C9 reacts with C-terminal pentapeptide of insulin β-chain. MAbs 7F8, D4B8, 3A6 and 8E2
react with different antigenic determinants.
These antibodies cross-react with human proinsulin, bovine insulin (30%) and porcine insulin. No cross-reaction with free C-peptide.
IgG1, kappa for all MAbs
Detection of insulin in two-site enzyme immunoassay. Following pairs are recommended (capture - detection):
7F8 - D4B8, 3A6 - 8E2.
MAb D4B8 can be used in immunohistochemistry on frozen sections.
Chromatography on protein A Sepharose
PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
+4 °C (+2 … +8 °C allowed)
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.