4IGF4
IBP3cc
IBP144, IBP154, IBP163, IBP180, IBP182, IBP185, IBP190,
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with recombinant human IGFBP-4 expressed in mammalian cell line (MAbs IBP144, IBP154, IBP180, IBP182, IBP185, IBP190), synthetic peptide from human IGFBP-4 His142-Met156 (MAbs IBP3cc) or synthetic peptide from human IGFBP-4 Lys157-Gln171 (MAb IBP163)
MAbs IBP144, IBP154 and IBP180: human IGFBP-4; human N-terminal IGFBP-4 fragment (NT-IGFBP-4)
MAbs IBP182, IBP185 and IBP190: human IGFBP-4; human C-terminal IGFBP-4 fragment (CT-IGFBP-4)
MAbs IBP3cc: human NT-IGFBP-4, cross-reaction with full-length human IGFBP-4 <5% (ELISA)
MAb IBP163: human CT-IGFBP-4, cross-reaction with full-length human IGFBP-4 <5% (ELISA)
IgG1 for MAbs IBP163, IBP190
IgG2a for MAbs IBP144, IBP154, IBP180
IgG2b for MAbs IBP182, IBP185
IgG3 for MAbs IBP3, IBP3cc
Detection of human IGFBP-4, NT-IGFBP-4, and CT-IGFBP-4. MAbs are working in direct ELISA and sandwich immunoassay.
Recommended pairs for human IGFBP-4 sandwich immunoassay (capture-detection):
IBP185 – IBP154
IBP182 – IBP144
IBP144 – IBP190
Recommended pairs for specific human NT-IGFBP-4 sandwich immunoassay (capture-detection):
IBP3cc – IBP144
IBP3cc – IBP180
Recommended pairs for specific human CT-IGFBP-4 sandwich immunoassay (capture-detection):
IBP182 – IBP163
Protein A chromatography
PBS, pH 7.4, 0.09 % sodium azide (NaN₃) for MAbs IBP144, IBP154, IBP163, IBP180, IBP182, IBP185, IBP190
50 mM citrate, 150 mM NaCl, pH 6.0, 0,09% azide (NaN₃) for MAb IBP3cc
+4 °C (+2 … +8 °C allowed)
This product is sold for research or further manufacturing use only. Standard Laboratory Practices should be followed when handling this material.
Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
(Catalogue number and MAb: 4IGF4-)
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