F8, InA108, InA245, InA180, InA224
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunised with purified influenza virus type A strain H1N1.
Influenza virus type A (nucleoprotein).
All MAbs detect NP of Influenza A with high specificity and have no cross reactivity to NP of Influenza B virus.
IgG1 for MAbs InA108 and InA224
IgG2a for MAb F8
IgG2b for MAb InA245
IgG3 for MAb InA180
MAb F8 can be used in immunocytochemistry. MAb F8 inhibits viral reproduction after fatty acid acylation.
MAbs InA108 and InA245 detect Influenza A nucleoprotein in Western blotting.
All MAbs are working in ELISA. Recommended pairs for Influenza A nucleoprotein in sandwich ELISA immunoassay (capture - detection): InA108 – InA245, InA224 – InA245.
Protein G chromatography for MAb F8
Protein A chromatography for MAbs InA108, InA245, InA180 and InA224
PBS, pH 7.4, 0.09 % sodium azide (NaN₃) for MAbs F8, InA108, InA245, InA224
50 mM citrate, 150 mM NaCl, pH 6.0, 0,09% azide (NaN₃) for MAb InA180
+4 °C (+2 … +8 °C allowed)
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.