Purification is conducted from broken yeast cells by following scheme: clarification, ammonium sulfate precipitation, ultrafiltration, cation exchange chromatography, zonal centrifugation, size-exclusion chromatography, sterile filtration.
20 mM KH2PO4 (pH 7.2), 500 mM NaCl, 1.7 mM EDTA, 0.01% Tween-80, 1 mM 2-Mercaptoethanol.
Purity > 90 % (SDS-PAGE).
Each lot is tested for protein content (Lowry method), lipid content (Total lipid), purity (SDS-PAGE), carbohydrate content (Anthrone method), antigen content (Immunoblot).
Tested in quantitative immunoblot with anti-HPVL1 antibodies.
-20 °C (-15 … -30 °C allowed)
Avoid repeated freezing and thawing.
It is recommended to aliquot the product after the first thawing.
This product is sold for research or further manufacturing use only. Standard Laboratory Practices should be followed when handling this material.
(Catalogue number and MAb: 8HPV18)