Human osteocalcin, antibody

Cat.# 4OC8

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Miscellaneous
  • Cat.#

    4OC8

  • MAbs:

    3G7, 1C4, 1C7, 3G8, 2H9, 6F9, 8H12

    Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with bovine osteocalcin with keyhole limpet hemocyanin and recombinant human osteocalcin with glutathione S-transferase.

  • Specificity:

    MAb 2H9 cross-reacts with bovine, rat and pig OC and is not cross-reacting with mouse OC.

    MAb 6F9 is not cross-reacting with bovine, rat, mouse, rabbit or pig OC.

    See the PDF file at the bottom of the page for more information.

  • MAb isotypes:

    IgG1 for MAbs 1C4, 1C7, 3G8, 6F9, 8H12
    IgG2a for MAb 2H9
    IgG2b for MAb 3G7

  • Applications:

    MAbs are working in ELISA. Recommended pairs are (capture-detection):

    2H9 - 6F9 (detects the large NH2- terminal fragment and intact hOC)
    3G8 - 2H9 (detects only the intact hOC).

  • Purification:

    Chromatography on protein A Sepharose

  • Presentation:

    PBS, pH 7.4, 0.09 % sodium azide (NaN3) for MAbs 6F9 and 2H9

    0.9 % NaCl, 0.09 % sodium azide (NaN3) for MAbs 3G7, 1C4, 1C7, 3G8 and 8H12

  • Storage:

    +4 °C (+2 … +8 °C allowed)

  • Material safety note:

    This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

    Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

  • References:

    Search references from PubMed

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Human osteocalcin, antibody (MAb: )

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