-
Cat.#
4OC8
-
MAbs in vitro:
2H9cc, 6F9cc, 1C4, 1C7, 3G8, 8H12 Mouse monoclonal antibody produced in bioreactor. Hybridoma clone derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice.
-
MAbs in vivo:
3G7 Mouse monoclonal antibody. Hybridoma clone derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice.
-
Immunogen:
Bovine osteocalcin with keyhole limpet hemocyanin for 3G7, 1C4, 1C7, 3G8Recombinant human osteocalcin with glutathione S-transferase for 2H9cc, 6F9cc, 8H12
-
Specificity:
2H9cc cross-reacts with bovine, rat and pig OC and is not cross-reacting with mouse OC. 6F9cc is not cross-reacting with bovine, rat, mouse, rabbit or pig OC. The ability of the MAbs to recognize full length human osteocalcin, tryptic 1-19, 20-43, synthetic 7-19, 15-31 and bovine osteocalcin was tested with Eu-labelled antigens. *3G8 recognizes unlabelled human osteocalcin (hOC) and bovine osteocalcin (bOC) when tested with two-site combinations.
-
MAb isotypes:
IgG1 for 1C4, 1C7, 3G8, 6F9cc, 8H12IgG2a for 2H9ccIgG2b for 3G7
-
Applications:
MAbs are working in ELISA. Recommended pairs are:
-
Purification:
Protein A chromatography
-
Presentation:
PBS, pH 7.4, 0.09 % sodium azide (NaN3 ) for 6F9cc, 2H9cc50 mM sodium citrate, 150 mM NaCl, pH 6.0, 0.09 % sodium azide (NaN3 ) for 1C4, 1C7, 3G8, 8H120.9 % NaCl, 0.09 % sodium azide (NaN3 ) for 3G7
-
Storage:
+4 °C (+2 … +8 °C allowed)
-
Material safety note:
This product is sold for research or further manufacturing use only. Standard Laboratory Practices should be followed when handling this material. Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
