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Monoclonal mouse anti-human osteocalcin Cat.#

4OC8

Data Sheet

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Properties
Unit

mg

Cat.#

4OC8

MAbs:

3G7, 1C4, 1C7, 3G8, 2H9, 6F9, 8H12

Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with bovine osteocalcin with keyhole limpet hemocyanin and recombinant human osteocalcin with glutathione S-transferase.

Specificity:

MAb 2H9 cross-reacts with bovine, rat and pig OC and is not cross-reacting with mouse OC.

MAb 6F9 is not cross-reacting with bovine, rat, mouse, rabbit or pig OC.

See the PDF file at the bottom of the page for more information.

MAb isotypes:

IgG1 for MAbs 1C4, 1C7, 3G8, 6F9, 8H12
IgG2a for MAb 2H9
IgG2b for MAb 3G7

Applications:

MAbs are working in ELISA. Recommended pairs are (capture-detection):

2H9 - 6F9 (detects the large NH2- terminal fragment and intact hOC)
3G8 - 2H9 (detects only the intact hOC).

Purification:

Chromatography on protein A Sepharose

Presentation:

PBS, pH 7.4, 0.09 % sodium azide (NaN3) for MAbs 6F9 and 2H9

0.9 % NaCl, 0.09 % sodium azide (NaN3) for MAbs 3G7, 1C4, 1C7, 3G8 and 8H12

Storage:

+4 °C (+2 … +8 °C allowed)

Material safety note:

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

Additional product information:

Osteocalcin TechNotes

References:

Search references from PubMed

Download datasheet as a PDF file below
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