3G7, 1C4, 1C7, 3G8, 8H12
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with bovine osteocalcin with keyhole limpet hemocyanin and recombinant human osteocalcin with glutathione S-transferase.
MAb 2H9cc cross-reacts with bovine, rat and pig OC and is not cross-reacting with mouse OC.
MAb 6F9cc is not cross-reacting with bovine, rat, mouse, rabbit or pig OC.
See the PDF file at the bottom of the page for more information.
IgG1 for MAbs 1C4, 1C7, 3G8, 6F9cc, 8H12
IgG2a for MAb 2H9cc
IgG2b for MAb 3G7
MAbs are working in ELISA. Recommended pairs are (capture-detection):
2H9cc - 6F9cc (detects the large NH2- terminal fragment and intact hOC)
3G8 - 2H9cc (detects only the intact hOC).
Protein A chromatography
PBS, pH 7.4, 0.09 % sodium azide (NaN3) for MAbs 6F9cc and 2H9cc
0.9 % NaCl, 0.09 % sodium azide (NaN3) for MAbs 3G7, 1C4, 1C7, 3G8 and 8H12
+4 °C (+2 … +8 °C allowed)
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.