Monoclonal mouse anti-hepatitis B virus surface antigen (HBsAg) Cat.#

3HB12

Data Sheet

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MAbs
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Properties
Unit

mg
Cat.#

3HB12
MAbs:

HB5, HB6, HB11, HB12, HB13, Hs33, Hs41

Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with recombinant HBsAg of ayw subtype.
Specificity:

HBsAg

MAbs are reacting with the following HBsAg subtypes: ayw1, ayw2, ayw3, ayw4, ayr, adw2, adw4, adrq+, adrq-, ayw3 (Fer).
MAb isotypes:

IgG1 for MAbs HB11, HB13, HB12, HB6, HB5, Hs41
IgG2a for MAb Hs33
Applications:

Detection of HBsAg in two-site enzyme immunoassay.

Recommended pairs for sandwich immunoassay:
HB12 - HB11, HB5 - HB6, HB13 - HB6, HB12 - HB6, Hs33 - Hs41

Best pair: Hs33 - Hs41, sensitivity 50 pg/ml
Purification:

Chromatography on protein G Sepharose.
Presentation:

PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
Storage:

+4 °C (+2 … +8 °C allowed)
Material safety note:

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
References:

Search references from PubMedﾠ.
Download datasheet as a PDF file below
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