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Monoclonal mouse anti-human chorionic gonadotropin (HCG) Cat.#

2H8

Data Sheet

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Properties
Unit

mg

Cat.#

2H8

MAbs:

28A4, 27E8,ᅠ 77F12, F1

Specificity:

MAbs 28A4 and 27E8 specifically recognize beta-subunit of HCG. MAbs are not cross-reacting with hLH, hTSH, hFSH.

MAb 77F12 specifically recognizes HCG; it reacts mainly with alpha-subunit. MAb is cross-reacting with whole molecule of HCG, hLH, hTSH and hFSH. Cross-reactivity with free beta-subunit of HCG was not detected.

MAb F1 is specific for the alpha-chain of FSH, LH, TSH and HCG.

MAb isotypes:

IgG1 for MAbs 27E8, F1
IgG2a for MAb 28A4
IgG2b for MAbs 77F12

Applications:

Detection of HCG in immunoassays. Suitable for immunochromatography (not tested with MAb F1).

Best recommended pairs for sandwich immunoassay (capture-detection): 77F12-28A4, 27E8-28A4

Purification:

Ion exchange chromatography for MAb F1.

Chromatography on protein A Sepharose for MAbs 28A4, 27E8 and 77F12.

Presentation:

0.15 M NaCl, 10 mM Tris, pH 7.5, 0.05% sodium azide (NaN) for MAb F1.

PBS, pH 7.4, 0.09 % sodium azide (NaN) for MAbs 28A4, 27E8 and 77F12.

Storage:

+4 °C (+2 … +8 °C allowed)

Material safety note:

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

Download datasheet as a PDF file below