Monoclonal mouse anti-human chorionic gonadotropin (HCG) Cat.#

2H8

Data Sheet

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MAbs
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Properties
Unit

mg
Cat.#

2H8
MAbs:

28A4, 27E8, 77F12, F1
Specificity:

MAbs 28A4 and 27E8 specifically recognize beta-subunit of HCG. MAbs are not cross-reacting with hLH, hTSH, hFSH.

MAb 77F12 specifically recognizes HCG; it reacts mainly with alpha-subunit. MAb is cross-reacting with whole molecule of HCG, hLH, hTSH and hFSH. Cross-reactivity with free beta-subunit of HCG was not detected.

MAb F1 is specific for the alpha-chain of FSH, LH, TSH and HCG.
MAb isotypes:

IgG1 for MAbs 27E8, F1
IgG2a for MAb 28A4
IgG2b for MAbs 77F12
Applications:

Detection of HCG in immunoassays. Suitable for immunochromatography (not tested with MAb F1).

Best recommended pairs for sandwich immunoassay (capture-detection): 77F12-28A4, 27E8-28A4
Purification:

Ion exchange chromatography for MAb F1.

Chromatography on protein A Sepharose for MAbs 28A4, 27E8 and 77F12.
Presentation:

0.15 M NaCl, 10 mM Tris, pH 7.5, 0.05% sodium azide (NaN₃) for MAb F1.

PBS, pH 7.4, 0.09 % sodium azide (NaN₃) for MAbs 28A4, 27E8 and 77F12.
Storage:

+4 °C (+2 … +8 °C allowed)
Material safety note:

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
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