Mouse monoclonal antibody produced in bioreactor. Hybridoma clone derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice.
28A4, 27E8, 77F12
Mouse monoclonal antibody produced in ascites. Hybridoma clone derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice.
Purified hCG alpha chain for F1cc
Native human HCG (whole molecule) for 28A4, 27E8, 77F12
MAbs 28A4 and 27E8 specifically recognize beta-subunit of HCG. MAbs are not cross-reacting with hLH, hTSH, hFSH.
MAb 77F12 specifically recognizes HCG; it reacts mainly with alpha-subunit. MAb is cross-reacting with whole molecule of HCG, hLH, hTSH and hFSH. Cross-reactivity with free beta-subunit of HCG was not detected.
MAb F1cc is specific for the alpha-chain of FSH, LH, TSH and HCG.
IgG1 for MAbs 27E8, F1cc
IgG2a for MAb 28A4
IgG2b for MAbs 77F12
Detection of HCG in immunoassays. Suitable for immunochromatography (not tested with F1cc).
Best recommended pairs for sandwich immunoassay (capture-detection):
77F12 - 28A4, 27E8 - 28A4
Protein A chromatography
PBS, pH 7.4, 0.09 % sodium azide (NaN3)
+4 °C (+2 … +8 °C allowed)
This product is sold for research or further manufacturing use only. Standard Laboratory Practices should be followed when handling this material.
Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
(Catalogue number and MAb: 2H8-)