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2H8
28A4, 27E8, 77F12, F1
MAbs 28A4 and 27E8 specifically recognize beta-subunit of HCG. MAbs are not cross-reacting with hLH, hTSH, hFSH.
MAb 77F12 specifically recognizes HCG; it reacts mainly with alpha-subunit. MAb is cross-reacting with whole molecule of HCG, hLH, hTSH and hFSH. Cross-reactivity with free beta-subunit of HCG was not detected.
MAb F1 is specific for the alpha-chain of FSH, LH, TSH and HCG.
IgG1 for MAbs 27E8, F1
IgG2a for MAb 28A4
IgG2b for MAbs 77F12
Detection of HCG in immunoassays. Suitable for immunochromatography (not tested with MAb F1).
Best recommended pairs for sandwich immunoassay (capture-detection): 77F12-28A4, 27E8-28A4
Ion exchange chromatography for MAb F1.
Chromatography on protein A Sepharose for MAbs 28A4, 27E8 and 77F12.
0.15 M NaCl, 10 mM Tris, pH 7.5, 0.05% sodium azide (NaN₃) for MAb F1.
PBS, pH 7.4, 0.09 % sodium azide (NaN₃) for MAbs 28A4, 27E8 and 77F12.
+4 °C (+2 … +8 °C allowed)
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
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