Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with human GPBB.
MAb 1G6 is reacting with BB isoenzyme. MAb 6F1 is reacting with BB and MM isoenzymes.
IgG1 for MAb 6F1
IgG2b for MAb 1G6
GPBB immunoassay and immunoaffinity purification.
All MAbs are working in Western blotting.
Recommended pair for sandwich immunoassay (capture - detection): 1G6 - 6F1
Important note: it is recommended to use three-step assay, since signal is low in one-step and two-step assays.
Chromatography on protein A Sepharose
PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
+4 °C (+2 … +8 °C allowed)
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.