Expressed in E. coli
Synthetic DNA fragment encoding amino acids 60-383 of human GFAP (Accession number UniProtKB P14136, GFAP_HUMAN) was used for recombinant protein expression.
Theoretical molecular weight 37 906 Da
Theoretical pI 4.95
Calibrator and standard preparation
Purity >90% (SDS-PAGE in reducing conditions)
Concentration is determined spectrophotometrically at A280
Ion exchange chromatography
Lyophilized from 16 mM NaHCO3, containing 0.05% CHAPS and 0.05% Tween 20.
It is recommended to reconstitute this product with deionized water to its initial concentration.
Lyophilized and reconstituted at -70°C (-65 … -80 °C allowed)
Avoid repeated freezing and thawing. It is recommended to aliquot the product after reconstitution.
The storage of lyophilized protein at -20°C may lead to a loss of 20% of immunochemical activity over two years.
For protein dilutions, we do not recommend PBS or other buffers with high ionic strength. This may lead to precipitation.
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.