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Hybridoma clone for MAb T14 has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with pooled water-soluble antigens from disintegrated microbial mass of Fr. tularensis vaccine strain.
Hybridoma clone for MAb FB11 has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with Fr. tularensis.
MAb T14 reacts with LPS of Fr. tularensis. There is no cross-reactivity with Y. pestis, Y. pseudotuberculosis, Y. enterocolitica, V. cholera, E. coli, S. typhimurium, Fr. novicida, Br. melitensis, Br. abortus, Br. suis, Br. ovis, and Br. neotomae.
MAb FB11 recognizes LPS of virulent and vaccine strains of Fr. tularensis. There is no crossreactivity with Fr. novicida, Br. abortus, Br. suis, Br. melitensis, Br. ovis, Y. pestis, Y. enterocolitica, Y. pseudotuberculosis, E. coli, and V. cholerae.
The binding site for MAb FB11 is located on the O-antigen polysaccharide chain which consists of tetrasaccharide fragments and has the following structure: -4)alpha-D-GalpNAcAN-(1-4)-alpha-DGalpNAcAN-( 1-3)-beta-D-QuipNAc-(1-2)-beta-Quip4NFm-(1.
Tetrasaccharide D-GalpNAcAN-(1-4)-alpha-D-GalpNAcAN-(1-3)-beta-D-QuipNAc-(1-2)-beta-D-Quip4NFm and trisaccharide D-GalpNAcAN-(1-3)-beta-D-QuipNAc-(1-2)-beta-D-Quip4NFm compete in ELISA for binding MAb FB11 with LPS of Fr. tularensis.
IgG2a for MAbs FB11
MAbs T14 and FB11 can be used for detection of Fr. tularensis in ELISA and immunofluorescence technique.
Chromatography on protein G Sepharose
PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
+4 °C (+2 … +8 °C allowed)
|Material safety note:||
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- 3FT6_DS.pdf (pdf)