Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with fibrinopeptide A conjugated with a carrier protein.
Fibrinopeptide A region in the alpha-chain of fibrinogen and free fibrinopeptide A.
IgG2a for MAbs 1F7 and 49D2
Fibrinogen and fibrinopeptide A immunoassay.
For specific fibrinogen detection in sandwich ELISA we recommend using anti-fibrinopeptide A MAbs for coating and anti-fibrinogen MAbs for conjugation.
Recommended pairs (capture - detection):
49D2 (anti-fibrinopeptide A) - 40F11 (anti-fibrinogen)
1F7 (anti-fibrinopeptide A) - 1F3 (anti-fibrinogen)
MAbs are working also in Western blotting.
Protein A chromatography
PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
+4 °C (+2 … +8 °C allowed)
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.