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Monoclonal mouse anti-Diphtheria toxin, A-subunit Cat.#

2DT14

Data Sheet

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MAbs
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Properties
Unit

mg
Cat.#

2DT14
MAbs:

8A4, 7F2

Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with Diphtheria toxoid.
Specificity:

8A4 reacts with free A-subunits of Diphtheria toxin. It does not react with the whole Diphtheria toxin and free B-subunit of Diphtheria toxin.

7F2 reacts with the epitope exposed on free A-subunit and on whole Diphtheria toxin molecule. It does not react with free B-subunit of Diphtheria toxin.
MAb isotypes:

IgG1 for MAb 7F2
IgG2a for MAb 8A4
Applications:

Detection of Diphtheria toxin A-subunit by different immunochemical techniques.

Recommend pair for sandwich immunoassay (capture - detection): 8A4 - 7F2.
Purification:

Chromatography on protein A Sepharose
Presentation:

PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
Storage:

+4 °C (+2 … +8 °C allowed)
References:

Search references from PubMed
Material safety note:

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Download datasheet as a PDF file below
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