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Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with Diphtheria toxoid.
8A4 reacts with free A-subunits of Diphtheria toxin. It does not react with the whole Diphtheria toxin and free B-subunit of Diphtheria toxin.
7F2 reacts with the epitope exposed on free A-subunit and on whole Diphtheria toxin molecule. It does not react with free B-subunit of Diphtheria toxin.
IgG1 for MAb 7F2
Detection of Diphtheria toxin A-subunit by different immunochemical techniques.
Recommend pair for sandwich immunoassay (capture - detection): 8A4 - 7F2.
Chromatography on protein A Sepharose
PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
+4 °C (+2 … +8 °C allowed)
|Material safety note:||
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
Search references from PubMed
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- 2DT14_DS.pdf (pdf)