Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with Diphtheria toxoid.
8A4 reacts with free A-subunits of Diphtheria toxin. It does not react with the whole Diphtheria toxin and free B-subunit of Diphtheria toxin.
7F2 reacts with the epitope exposed on free A-subunit and on whole Diphtheria toxin molecule. It does not react with free B-subunit of Diphtheria toxin.
IgG1 for MAb 7F2
IgG2a for MAb 8A4
Detection of Diphtheria toxin A-subunit by different immunochemical techniques.
Recommend pair for sandwich immunoassay (capture - detection): 8A4 - 7F2.
Protein A chromatography
PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
+4 °C (+2 … +8 °C allowed)
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.