D-dimer human, antibody

4D30

DD3cc, DD6cc, DD41cc, DD44cc, DD46cc, DD189cc, DD255cc

Mouse monoclonal antibody produced in bioreactor. Hybridoma clone derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice.

DD1, DD2, DD4, DD5, DD22, DD93

Mouse monoclonal antibody produced in ascites. Hybridoma clone derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice.

D-dimer for DD1, DD189cc, DD2, DD255cc, DD3cc, DD4, DD5, DD6cc

Mixture of D-dimer and high molecular weight fibrin degradation products for DD22, DD41cc, DD44cc, DD46cc

Synthetic peptides covering the cross-linked region of D-dimer gamma-chain for DD93

D-dimer and high molecular weight fibrin degradation products, cross-reactivity with fibrinogen for DD4, DD5, DD6cc.

D-dimer and high molecular weight fibrin degradation products, no cross-reactivity with fibrinogen for DD1, DD189cc, DD2, DD22, DD255cc, DD3cc, DD41cc, DD44cc, DD46cc.

D-dimer, high molecular weight fibrin degradation products and a cross-linked region of D-dimer, no cross-reactivity with fibrinogen for DD93.

IgG1 for DD93, DD189cc, DD255cc
IgG2a for DD1, DD6cc, DD22, DD41cc, DD46cc
IgG2b for DD2, DD3cc, DD4, DD5, DD44cc

Immunoassays for the quantitative determination of D-dimer and high molecular weight fibrin degradation products. MAbs recognize D-dimer in ELISA.

*Due to the cross-reactivity of DD4 with fibrinogen, we strongly recommend using it as the detection antibody. In a sandwich immunoassay, plasma must be diluted at least two-fold with 10 mM Tris-HCl, pH 7.5, 1 M NaCl, 0.1 % Tween 20 to avoid nonspecific binding. Each step in the assay should be followed by an incubation and wash: coating with the capture MAb, addition of the sample and addition of the (conjugated) detection MAb.
MAbs recognize D-dimer in Western blotting under non-reducing conditions.
DD22, DD41cc, DD44cc, DD46cc and DD189cc interact with beta-chain of D-dimer in Western blotting under reducing conditions.
DD93 and DD255cc interact with gamma-chain of D-dimer in Western blotting under reducing conditions.

Protein A chromatography

PBS, pH 7.4, 0.09 % sodium azide (NaN₃)

+4 °C (+2 … +8 °C allowed)

This product is sold for research or further manufacturing use only. Standard Laboratory Practices should be followed when handling this material.

Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.