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Monoclonal mouse anti-D-dimer Cat.#

4D30

Data Sheet

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Properties
Cat.#

4D30

Unit

mg

MAbs:

DD1, DD2, DD3, DD4, DD5, DD6, DD22, DD41, DD44, DD46, DD93, DD189, DD255

Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with homogenized fibrin clot, D-dimer or high molecular weight fibrin degradation products.

Specificity:

All MAbs recognize D-dimer and high molecular weight fibrin degradation products.

MAb DD93 recognizes a cross-linked region of D-dimer.

MAbs DD1, DD2, DD3, DD22, DD41, DD44, DD46, DD93, DD189 and DD255 do not cross-react with fibrinogen.

MAbs DD4, DD5 and DD6 show cross-reaction with fibrinogen.

MAb isotypes:

IgG1 for MAbs DD93, DD189, DD255
IgG2a for MAbs DD1, DD6, DD22, DD41, DD46
IgG2b for MAbs DD2, DD3, DD4, DD5, DD44.

Applications:

Immunoassays for the quantitative determination of D-dimer and high molecular weight fibrin degradation products.

All antibodies recognize D-dimer in ELISA. All MAbs recognize D-dimer in Western blotting under non-reducing conditions. MAbs DD22, DD41, DD44, DD46 and DD189 interact with beta-chain of D-dimer in Western blotting under reducing conditions. MAbs DD93 and DD255 interact with gamma-chain of D-dimer in Western blotting under reducing conditions.

Recommended pairs to be used in a sandwich immunoassay for D-dimer detection in human plasma (capture-detection):

DD189 – DD255 (Equal specificity for D-dimer and high MW fibrin degradation products).

DD2 – DD41 (Slightly more specific for high MW fibrin degradation products).

DD2 – DD4* (Approximately equal specificity for D-dimer and high MW fibrin degradation products).

(*Due to the cross-reactivity of DD4 with fibrinogen, we strongly recommend to use it as the detection antibody. In a sandwich immunoassay, plasma must be diluted at least two-fold with 10 mM Tris-HCl, pH 7.5, 1 M NaCl, 0.1 % Tween 20 in order to avoid nonspecific binding. Each step in the assay should be followed by an incubation and wash: coating with the capture MAb, addition of the sample and addition of the (conjugated) detection MAb.)

Purification:

Chromatography on protein A Sepharose

Presentation:

PBS, pH 7.4, 0.09 % sodium azide (NaN)

Storage:

+4 °C (+2 … +8 °C allowed)

Material safety note:

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

Additional product information:

D-dimer and High Molecular Weight Fibrin Degradation Products TechNotes
D-dimer product info

References:

See all D-dimer references.
Search other references from PubMed.

Download datasheet as a PDF file below