D-dimer human, antibody

Cat.# 4D30

This data sheet is provided for information only. After adding the product to the cart by clicking the button below you will see if there is a volume discount available.

Sign up or login to see prices and to request offers

MAbs:
Price:
-- / mg
* All prices excluding VAT
Request an offer
Blood coagulation and anemia
  • Cat.#

    4D30

  • MAbs in vivo:

    DD1, DD2, DD3, DD4, DD5, DD6, DD22, DD41, DD44, DD46, DD93, DD189, DD255

    Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with D-dimer, high molecular weight fibrin degradation products or synthetic peptides covering the cross-linked region of D-dimer gamma-chain.

  • MAbs in vitro:

    DD41cc

    Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with D-dimer, high molecular weight fibrin degradation products or synthetic peptides covering the cross-linked region of D-dimer gamma-chain.

  • Specificity:

    All MAbs recognize D-dimer and high molecular weight fibrin degradation products.

    MAb DD93 recognizes a cross-linked region of D-dimer.

    MAbs DD1, DD2, DD3, DD22, DD41, DD41cc DD44, DD46, DD93, DD189 and DD255 do not cross-react with fibrinogen.

    MAbs DD4, DD5 and DD6 show cross-reaction with fibrinogen.

  • MAb isotypes:

    IgG1 for MAbs DD93, DD189, DD255
    IgG2a for MAbs DD1, DD6, DD22, DD41, DD41cc DD46
    IgG2b for MAbs DD2, DD3, DD4, DD5, DD44.

  • Applications:

    Immunoassays for the quantitative determination of D-dimer and high molecular weight fibrin degradation products

    All antibodies recognize D-dimer in ELISA. All MAbs recognize D-dimer in Western blotting under non-reducing conditions. MAbs DD22, DD41, DD41cc, DD44, DD46 and DD189 interact with betachain of D-dimer in Western blotting under reducing conditions. MAbs DD93 and DD255 interact with gamma-chain of D-dimer in Western blotting under reducing conditions.

    Recommended pairs to be used in a sandwich immunoassay for D-dimer detection in human plasma (capture-detection):

    DD189 – DD255 (Equal specificity for D-dimer and high MW fibrin degradation products).

    DD2 – DD41cc (Slightly more specific for high MW fibrin degradation products).

    DD2 – DD4* (Approximately equal specificity for D-dimer and high MW fibrin degradation products).

    (*Due to the cross-reactivity of DD4 with fibrinogen, we strongly recommend to use it as the detection antibody. In a sandwich immunoassay, plasma must be diluted at least two-fold with 10 mM Tris-HCl, pH 7.5, 1 M NaCl, 0.1 % Tween 20 in order to avoid nonspecific binding. Each step in the assay should be followed by an incubation and wash: coating with the capture MAb, addition of the sample and addition of the (conjugated) detection MAb.)

  • Purification:

    Chromatography on protein A Sepharose

  • Presentation:

    PBS, pH 7.4, 0.09 % sodium azide (NaN)

  • Storage:

    +4 °C (+2 … +8 °C allowed)

  • Material safety note:

    This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

    Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

  • References:

    See all D-dimer references.
    Search other references from PubMed.

×

Bulk Price Quotation

D-dimer human, antibody (MAb: )

Information

Sign up to our newsletter

And be informed about new products and scientific findings

Thank you for your subscription!