D-dimer human, antibody

4D30

RD15
Recombinant monoclonal antibody expressed in a mammalian cell line.

DD3cc, DD6cc, DD41cc, DD44cc, DD46cc, DD189cc, DD255cc

Mouse monoclonal antibody produced in bioreactor. Hybridoma clone derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice.

DD1, DD2, DD4, DD5, DD22, DD93

Mouse monoclonal antibody produced in ascites. Hybridoma clone derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice.

D-dimer for RD15, DD1, DD189cc, DD2, DD255cc, DD3cc, DD4, DD5, DD6cc

Mixture of D-dimer and high molecular weight fibrin degradation products for DD22, DD41cc, DD44cc,
DD46cc

Synthetic peptides covering the cross-linked region of D-dimer gamma-chain for DD93

D-dimer and high molecular weight fibrin degradation products, cross-reactivity with fibrinogen for
DD4, DD5, DD6cc.

D-dimer and high molecular weight fibrin degradation products, no cross-reactivity with fibrinogen for
RD15, DD1, DD189cc, DD2, DD22, DD255cc, DD3cc, DD41cc, DD44cc, DD46cc.

D-dimer, high molecular weight fibrin degradation products and a cross-linked region of D-dimer, no
cross-reactivity with fibrinogen for DD93.

IgG1 for RD15, DD93, DD189cc, DD255cc
IgG2a for DD1, DD6cc, DD22, DD41cc, DD46cc
IgG2b for DD2, DD3cc, DD4, DD5, DD44cc

Immunoassays for the quantitative determination of D-dimer and high molecular weight fibrin
degradation products. All MAbs recognize D-dimer in ELISA.

*Due to the cross-reactivity of DD4 with fibrinogen, we strongly recommend using it as the detection
antibody. In a sandwich immunoassay, plasma must be diluted at least two-fold with 10 mM Tris-HCl,
pH 7.5, 1 M NaCl, 0.1 % Tween 20 to avoid nonspecific binding. Each step in the assay should be
followed by an incubation and wash: coating with the capture MAb, addition of the sample and addition
of the (conjugated) detection MAb.

All MAbs recognize D-dimer in Western blotting under non-reducing conditions.

DD22, DD41cc, DD44cc, DD46cc and DD189cc interact with beta-chain of D-dimer in Western blotting
under reducing conditions.

DD93, RD15 and DD255cc interact with gamma-chain of D-dimer in Western blotting under reducing
conditions.

Protein A chromatography

50 mM sodium citrate, 150 mM NaCl, pH 6.0, 0.09 % sodium azide (NaN3) for RD15

PBS, pH 7.4, 0.09 % sodium azide (NaN3) for DD3cc, DD6cc, DD41cc, DD44cc, DD46cc, DD189cc,
DD255cc, DD1, DD2, DD4, DD5, DD22, DD93

+4 °C (+2 … +8 °C allowed)

This product is sold for research or further manufacturing use only. Standard Laboratory Practices should be followed when handling this material.

Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.