D-dimer human, antibody

4D30

DD3cc, DD6cc, DD41cc, DD44cc, DD46cc, DD189cc, DD255cc

DD1, DD2, DD4, DD5, DD22, DD93

Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with D-dimer, high molecular weight fibrin degradation products or synthetic peptides covering the cross-linked region of D-dimer gamma-chain.

All MAbs recognize D-dimer and high molecular weight fibrin degradation products.
DD93 recognizes a cross-linked region of D-dimer.
DD1, DD2, DD3cc, DD22, DD41cc, DD44cc, DD46cc, DD93, DD189cc and DD255cc do not cross-react with fibrinogen.
DD4, DD5 and DD6cc show cross-reaction with fibrinogen.

IgG1 for MAbs DD93, DD189cc, DD255cc
IgG2a for MAbs DD1, DD6cc, DD22, DD41cc, DD46cc
IgG2b for MAbs DD2, DD3cc, DD4, DD5, DD44cc

Immunoassays for the quantitative determination of D-dimer and high molecular weight fibrin degradation products
All antibodies recognize D-dimer in ELISA. All MAbs recognize D-dimer in Western blotting under non-reducing conditions. DD22, DD41cc, DD44cc, DD46cc and DD189cc interact with beta-chain of D-dimer in Western blotting under reducing conditions. DD93 and DD255cc interact with gamma-chain of D-dimer in Western blotting under reducing conditions.
Recommended pairs to be used in a sandwich immunoassay for D-dimer detection in human plasma, see pdf

* Due to the cross-reactivity of DD4 with fibrinogen, we strongly recommend to use it as the detection antibody. In a sandwich immunoassay, plasma must be diluted at least two-fold with 10 mM Tris-HCl, pH 7.5, 1 M NaCl, 0.1 % Tween 20 in order to avoid nonspecific binding. Each step in the assay should be followed by an incubation and wash: coating with the capture MAb, addition of the sample and addition of the (conjugated) detection MAb.

Protein A chromatography

PBS, pH 7.4, 0.09 % sodium azide (NaN₃)

+4 °C (+2 … +8 °C allowed)

This product is sold for research or further manufacturing use only. Standard Laboratory Practices should be followed when handling this material.

Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.