4CA30 / 4CA30cc
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with human natural tumor-derived purified CEA antigen.
3C8cc: epitope specificity group V
3C6: epitope specificity group I
3C1: epitope specificity group IVa or II
Epitope specificity is given per ISOBM classification. MAbs are not cross-reacting with human leukocytes in cytofluorometry.
IgG1 for 3C8cc, 3C1, 3C6
3C6 can be used in immunohistochemistry.
3C1 and 3C6 are working in EIA. MAbs 3C1 and 3C6 are working in Western Blotting.
Recommended pair for sandwich immunoassay (capture - detection): 3C6 - 3C1.
Protein A chromatography for 3C8cc
Ion exchange chromatography for 3C1, 3C6
PBS, pH 7.4, 0.09 % sodium azide (NaN3) for 3C8cc
10 mM Tris, pH 7.5, 0.15 M NaCl, 0.05 % sodium azide (NaN3) for 3C1, 3C6
+4 °C (+2 … +8 °C allowed)
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.