4C10 / 4C10cc
13G11cc, 14A2cc, 16B5cc, 24B2cc, P138, P139, P141
Recombinant chimeric antibody expressed in a mammalian cell line. Composed of original wild type variable domains of mouse derived MAb and human lgG1 constant domains (RC16B5).
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with calcitonin conjugated with carrier protein (13G11cc, 14A2cc, 16B5cc, 24B2cc, 13B9, 13F2) or recombinant human procalcitonin (P138, P139, P141).
Epitope specificity: see PDF
IgG1 for 24B2cc, 13F2, 13G11cc, 14A2cc, P138, P139, P141, RC16B5
IgG2a for 13B9
IgG2b for 16B5cc
Detection of human calcitonin in immunoassays.
Recommended pairs for sandwich immunoassay:
Recommended pairs for sandwich immunoassay (capture - detection): 13B9 - 13F2, 24B2cc - 13B9
MAbs 13G11cc, 14A2cc, 16B5cc, 24B2cc and 13F2 can be used for procalcitonin detection in Western blotting after SDS-electrophoresis in reducing conditions
Protein A chromatography
PBS, pH 7.4, 0.09 % sodium azide (NaN₃) for 13G11cc, 14A2cc, 16B5cc, 24B2cc, P138, P139, P141, 13B9, 13F2
50 mM sodium citrade, 150 mM NaCl, Ph6, 0,09% sodium azide (NaN3) for RC16B5
+4 °C (+2 … +8 °C allowed)
Please see also antibodies specific to other parts of procalcitonin under Cat.# 4PC47
This product is sold for research or further manufacturing use only. Standard Laboratory Practices should be followed when handling this material.
Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
(Catalogue number and MAb: 4C10 / 4C10cc-)