Monoclonal mouse anti-human calcitonin Cat.#

4C10 / 4C10cc

Data Sheet

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MAbs
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Properties
Unit

mg
Cat.#

4C10 / 4C10cc
MAbs in vivo (Cat.# 4C10):

13B9, 24B2, 13F2, 13G11, 14A2, 16B5

Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with calcitonin conjugated with carrier protein.
MAbs in vitro (Cat.# 4C10cc):

14A2cc, 16B5cc

Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with calcitonin conjugated with carrier protein.
Specificity:

Epitope specificity:
13B9: a.a.r. 60-69
24B2: a.a.r. 72-81
13F2: a.a.r. 72-81
13G11: a.a.r. 72-81
14A2, 14A2cc: a.a.r. 72-81
16B5, 16B5cc: a.a.r. 72-81
MAb isotypes:

IgG1 for MAbs 24B2, 13F2, 13G11, 14A2, 14A2cc
IgG2a for MAb 13B9
IgG2b for MAb 16B5, 16B5cc
Applications:

Detection of human calcitonin in ELISA.

All MAbs can also be used for procalcitonin detection in Western blotting after SDS-electrophoresis in reducing conditions.

Recommended pairs for sandwich immunoassay (capture - detection) : 13B9 - 13F2, 24B2 - 13B9
Purification:

Chromatography on protein A Sepharose
Presentation:

PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
Storage:

+4 °C (+2 … +8 °C allowed)
Other information:

Please see also antibodies specific to other parts of procalcitonin under Cat.# 4PC47, Monoclonal mouse anti-human procalcitonin (PCT)
Material safety note:

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Additional product information:

Procalcitonin TechNotes
Procalcitonin and Calcitonin Products
Antibodies Produced in vitro
Recombinant human procalcitonin antigen (Cat.# 8PC5)
References:

Search references from PubMed
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