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Monoclonal mouse anti-CA125 Cat.#

4C29

Data Sheet

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Properties
Unit

mg
Cat.#

4C29
MAbs:

X75,ﾠ X306, X325 and X52

Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with CA125 antigen purified from human ovarian carcinoma (MW > 1 MDa).
Specificity:

CA125 antigen.

MAbs X52; X75, X325: epitope specificity group B (ISOBM classification) similar to M-11.

MAb X306: epitope specificity group A (ISOBM classification) similar to OC125.
MAb isotypes:

IgG1 for all MAbs
Applications:

Detection of CA125 antigen. Suggested pairs for sandwich immunoassay (capture-detection):

X306 – X52
X306 – X325
X306 – X75

All MAbs are working in Western blotting. MAb X325 can be used in immunohistochemistry on paraffin embedded tissue (see pictures below).
Purification:

Ion exchange chromatography.
Presentation:

10 mM Tris, pH 7.5, 0.15 M NaCl, 0.05 % sodium azide (NaN₃)
Storage:

+4 °C (+2 … +8 °C allowed)
Material safety note:

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
References:

See other references.
Search CA-125 antibody references from PubMed.
Download datasheet as a PDF file below
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