Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with CA125 antigen purified from human ovarian carcinoma (MW > 1 MDa).
MAbs X52cc; X75, X325: epitope specificity group B (ISOBM classification) similar to M-11.
MAb X306cc: epitope specificity group A (ISOBM classification) similar to OC125.
IgG1 for all MAbs
Detection of CA125 antigen. Suggested pairs for sandwich immunoassay (capture-detection):
X306cc – X52cc
X306cc – X325
X306cc – X75
All MAbs are working in Western blotting. MAb X325 can be used in immunohistochemistry on paraffin embedded tissue.
Protein A chromatography for X306cc, X52cc
Ion exchange chromatography for X75, X325
PBS, pH 7.4, 0.09 % sodium azide (NaN₃) for X306cc, X52cc
10 mM Tris, pH 7.5, 0.15 M NaCl, 0.05 % sodium azide (NaN₃) for X75, X325
+4 °C (+2 … +8 °C allowed)
This product is sold for research or further manufacturing use only. Standard Laboratory Practices should be followed when handling this material.
Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
(Catalogue number and MAb: 4C29-)