CII-11, CII-29, CII-55, CC27, CC34
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with fragments of rat C-peptides I and II conjugated with the carrier protein
Info in the PDF file at the bottom of the page.
IgG1 for MAbs CII-11, CII-29, CII-55, CC27, CC34
All MAbs recognize rat C-peptide in ELISA and Sandwich type immunoassay.
MAbs can be used for separate or consistent detection of rat C-peptide isoforms (rat C-peptide I and/or rat C-peptide II) in biological samples.
For sandwich immunoassay the general recommendation is to use combinations of MAbs named as “CC” (MAbs specific to C-terminal part of rat C-peptide) and MAbs named as “CII” (MAbs specific to N-terminal part of rat C-peptide). The most sensitive pairs recommended for sandwich-type rat C-peptide immunoassays are (capture-detection):
Chromatography on protein A Sepharose
PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
+4 °C (+2 … +8 °C allowed)
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.