Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with B. anthracis spore extract.
MAbs SA26 and SA27 recognize 92-94K protein bands in Western blotting of B. anthracis spore extract. No cross-reactivity found in ELISA with B. anthracis vegetative cells, B. globigii, B. subtilis and B. cereus spores.
IgG2a for MAbs SA26 and SA27
MAb SA26 is suitable for coating and MAb SA27 could be used as a conjugate in sandwich ELISA for anthrax spore determination.
Both MAbs are working in Western blotting.
Chromatography on protein G Sepharose
PBS, pH 7.2, 0.09 % sodium azide (NaN₃)
+4 °C (+2 … +8 °C allowed)
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.