HeLa cells, inoculated with Adenovirus, type 6, strain Tonsil 99
Virus was grown on monolayer of HeLa cells. When the cultures showed pronounced c.p.e. virus containing medium was purified from cell fragments by low speed centrifugation (4000 rpm, 40 min). Virus was pelleted by high speed centrifugation at 18000 rpm for 2.5 hours in the SW27 rotor for 20 min at +4 °C, then the supernatant was collected and protein concentration was determined by Lowry method.
STE, 0.09 % sodium azide (NaN₃), 0.005 % thimerosal.
Thimerosal and beta propiolactone treatment
This product has been treated in a manner consistent with methods of inactivation. Generally accepted good laboratory practices appropriate to microbiological/viral safe handling practices and techniques are required at work.
Purity > 90 % (SDS-PAGE).
Tested with anti-adenovirus antibodies (Cat. 3AV13) in ELISA.
-20 °C (-15 … -30 °C allowed)
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
Product contains thimerosal and sodium azide as a preservative. Although the amounts are very small appropriate care must be taken when handling this product.